Nucleic Acid 208.5.1

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Leila Halaby Biochemistry 208.5.1 09/11/14 The Process of DNA Replication Western Governors University

The Process Of DNA Replication

The process of DNA replication at the biochemistry level starts with DNA to RNA and RNA to protein. DNA is “The Master” molecule of every cell. It contains vital information that is passed onto every generation. It has information on how to make itself as well as other molecules. DNA is the key to life. RNA leaves the nucleus of the cell and makes proteins. (Sally Seller December 2013)

http:/ /www.cytochemistry.net/cell-biology/ ribosome.htm

The process by which both strands are replicated starts with the strands being separated and unwound by DNA Helicase. DNA polymerases, an enzymes which catalyze the synthesis of new complementary strands will add nucleotides to the 3’ end of the growing nucleotide strand. An RNA primer is needed to start the synthesis of a new strand to make the first 3’ end the enzyme needs. After that, the RNA is taken out and replace with DNA. The synthesis of both complementary strands start in the 5’ to 3’ direction. The leading strand will then grow continuously in the direction of the replication fork. On the leading strand, RNA primase comes in and add RNA primer.

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(thinkwell biochemistry 6.4.2)

Newly synthesized DNA

The lagging strand will point away from the opening of the fork. RNA primase will constantly add RNA primer as the strand opens up On the leading strand, DNA polymerase III will work to add all the DNA nucleotide . On the lagging strand, DNA polymerase III will also be the main replication enzyme adding all the nucleotide, but instead of working continually toward the replication fork, it will be working away from the replication fork and will add the base pair needed at that time. DNA polymerase I will remove RNA primer and replaces it with DNA nucleotide. The…...

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